aureus strain V8 protease or proteinase K, due to the presence of a Pro residue at position P2 of the peptide. A Pro residue at this position seems to determine the loss of inhibitory activity ( Gebhard et al., 2004). The comparison selleck compound of the deduced amino acid sequence of contig PI01 and PSKP1 sequence showed that both proteins share this feature ( Fig. 2B). The singlet PI02 also showed the same characteristic, thus the encoded proteins from both groups seem to lack the inhibitory activity. Additionally we could find a potential signal peptide in both DNA sequences of these clusters,
using the software SignalP 4.0 (http://www.cbs.dtu.dk/services/SignalP/). On the other hand, the singlet PI03 showed low similarity (∼43%) to Kazal type 2 (GenBank ID:XP_002940646), a serine proteinase inhibitor, in which all conserved residues common to this family members were present in the deduced amino acid sequence of the singlet PI03, with a single exception for the Pro residue at position P1. This substitution is semi-conservative, but the effects on biological activity are not predictable since Pro residues impose important structural changes. Very limited information on structure-activity relationship was found in the literature
for this type of Kazal type 2 serine proteases inhibitors (Gebhard et al., 2004), suggesting the importance of conducting further pharmacological studies to better understand the structural requirements for this biological activity. The clusters encoding for sequences with similarity to cellular proteins represented Pexidartinib 64.3% of total ‘hit sequences’, and they were categorized according to the demonstrated or presumed biochemical functions and pathways (Table 2). Among these sequences, the most representative ESTs were those related to transcriptional and translational processes, covering about 20.7% of all analyzed contigs (Fig. 4). This group comprises ribosomal proteins, and transcription and elongation
factor homologues. The expression of these precursors is indicative of the high biosynthesis process in response to the continuous production of skin secretion. Transcripts encoding for proteins related to regulatory Janus kinase (JAK) processes represent the second most abundant group (14.2% of total). Amarked occurrence was observed for four transcripts encoding S100 calcium binding proteins. This large calcium binding protein family is composed by low molecular weight members with two different types of calcium binding domains. These proteins have an unusual calcium-binding EF-hand motif at the N-terminal portion, composed by a helix-loop-helix domains also referred as imperfect EF-hand motif with low affinity for calcium. The C-terminal portion has a conserved true EF-hand motif with high affinity to calcium.