gondii and Sarcocystis spp. There was no reactivity in the IHC test for T. gondii, even though a high number of Sarcocystis spp. was present in the conventional
H&E-stained histopathological sections of the heart. This data demonstrate the efficiency of this primary antibody. The IHC results in this study revealed that almost half of the animals positive by the MAT were possible sources of infection for humans because bradyzoites were identified in different tissues, regardless of MAT titration. However, with regard to the presence of T. gondii tissue cysts, there was a significant difference between the animals that had high titres and those with low titres for T. gondii in MAT. In animals that had high titres for T. gondii, cysts were found in the three evaluated organs – liver, heart and brain, whereas in animals with low titres, the cysts were observed only VRT752271 in vivo in the heart. This result suggests, C646 cell line that the heart is the organ of choice for the detection of bradyzoites by IHC in animals with low titres. Therefore, the IHC test was able to identify the dissemination of T. gondii as a zoonotic agent in the RJ State, suggesting that the consumption of ovine meat and organs may present an important source of infection for humans.
This could partially explain the high prevalence of human toxoplasmosis in this region of Brazil. We would like to thank Dr. J.P. Dubey, for kindly providing antigen for the MAT and Dra. Andréa Pires, for kindly providing the positive controls used for IHC. This study was supported by CAPES and FAPERJ. ”
“Toxoplasma gondii is a protozoan parasite that commonly affects not a wide range of birds and mammals, including humans ( Dubey and Beattie,
1988). Toxoplasmosis has been identified in many species of free-ranging and captive marine mammals such as sea lions, seals, walruses and manatees ( Dubey et al., 2003 and Dubey et al., 2009), southern sea otters (Enhydra lutris nereis) ( Conrad et al., 2005), whales ( Mazzariol et al., 2012) and several species of dolphins ( Inskeep et al., 1990, Migaki et al., 1990, Resendes et al., 2002, Dubey et al., 2003 and Dubey et al., 2009). Reports of T. gondii infection in aquatic mammals from Brazil are restricted to few studies such as a Guiana dolphin (Sotalia guianensis) stranded in the state of Rio de Janeiro ( Bandoli and Oliveira, 1977), and positive antibodies were found in free-living Amazon river dolphins (Inia geoffrensis) ( Santos et al., 2011) and captive Amazonian manatees (Trichechus inunguis) from the Brazilian Amazon ( Mathews et al., 2012). Guiana dolphin is a coastal species distributed from Honduras (15°58′N) in Central America down to the state of Santa Catarina (27°35′S) in Southern Brazil (Flores and da Silva, 2009). This dolphin inhabits estuaries, bays and shallow coastal waters and its conservation status is “data deficient” (IUCN, 2012).